Present-2013 2012-2008 2007-2005 2004-2002
2001 2000 1999
Crystal structure of cobalt-containing nitrile hydratase
A. Miyanaga, S. Fushinobu, K. Ito, and T. Wakagi
Biochem. Biophys. Res. Comm. 288 (5), 1169-1174 (2001)Structural basis for the ADP-specificity of a novel glucokinase from a hyperthermophilic archaeon
S. Ito, S. Fushinobu, I. Yoshioka, S. Koga, H. Matsuzawa, and T. Wakagi
Structure 9 (3), 205-214 (2001)Identification of the catalytic residue of Thermococcus litoralis 4-alpha-glucanotransferase through mechanism-based labeling
H. Imamura, S. Fushinobu, B. S. Jeon, T. Wakagi, and H. Matsuzawa
Biochemistry 40 (41), 12400-12406 (2001)Role of a highly conserved YPITP motif in 2-oxoacid : ferredoxin oxidoreductase - Heterologous expression of the gene from Sulfolobus sp strain 7, and characterization of the recombinant and variant enzymes
E. Fukuda, H. Kino, H. Matsuzawa, and T. Wakagi
Eur. J. Biochem. 268 (21), 5639-5646 (2001)HscA is involved in the dynamics of FtsZ-ring formation in Escherichia coli K12
T. Uehara, H. Matsuzawa, and A. Nishimura
Genes Cells 6 (9), 803-814 (2001)
Genes Cells 6 (11), 1017-1018 (2001) [Erratum]Some Lactobacillus L-lactate dehydrogenases exhibit comparable catalytic activities for pyruvate and oxaloacetate
K. Arai, T. Kamata, H. Uchikoba, S. Fushinobu, H. Matsuzawa, and H. Taguchi
J. Bacteriol. 183 (1), 397-400 (2001)
超好熱性古細菌 Thermococcus litoralis 由来4-α-グルカノトランスフェラーゼの反応機構と構造解析
（糖質関連酵素化学シンポジウム）
今村博臣、伏信 進矢、山本雅貴、熊坂崇、若木高善、松澤 洋
応用糖質科学 48 (2), 171-175 (2001)
Reaction mechanism and crystal structure of 4-alpha-glucanotransferase from a hyperthermophilic archaeon, Thermococcus litoralis
(Proceedings of the Symposium on Amylases and Related Enzymes, 2000)
H. Imamura, S. Fushinobu, M. Yamamoto, T. Kumasaka, T. Wakagi, and H. matsuzawa
J. Appl. Glycosci. 48 (2), 171-175 (2001) [Japanese]
Requirement for the COOH-terminal pro-sequence in the translocation of aqualysin I across the cytoplasmic membrane in Escherichia coli
D. W. Kim, and H. Matsuzawa
Biochem. Biophys. Res. Commun. 277 (1), 216-220 (2000)Application of a metal switch to aqualysin I, a subtilisin-type bacterial serine protease, to the S3 site residues, Ser102 and Gly131
T. Tanaka, Y. Kikuchi, H. Matsuzawa, and T. Ohta
Biosci. Biotechnol. Biochem. 64 (9), 2008-2011 (2000)
Ｘ線結晶解析と蛋白質工学による酵素の構造と機能に関する研究
（1999年度日本農芸化学会功績賞 受賞総説）
松澤洋
日本農芸化学会誌 74 (4), 478-499 (2000)
Studies on the Structure and Function of Enzymes by X-ray Crystallography and Protein Engineering
(Award Review of The Japan Bioscience, Biotechnology and Agrochemistry Society Senior Scientist Award, 1999)
Nippon Nogeikagaku Kaishi-Journal of the Japan Society for Bioscience Biotechnology and Agrochemistry 74 (4), 478-499 (2000)Structural basis for the ADP-specificity of a novel glucokinases from a hyperthermophilic archaeon
S. Ito, S. Fushinobu, and T. Wakagi
Photon Factory Activity Report #18 2000 Part A, Highlights [Biological Science], pp. 38
1999年度日本農芸化学会功績賞
Ｘ線結晶解析と蛋白質工学による酵素の構造と機能に関する研究
酵素学研究室教授 松沢 洋
The Japan Bioscience, Biotechnology and Agrochemistry Society Senior Scientist Award, 1999
Studies on the Structure and Function of Enzymes by X-ray Crystallography and Protein Engineering
Prof. Hiroshi Matsuzawa
Crystal structure of the E166A mutant of extended-spectrum beta-lactamase Toho-1 at 1.8A resolution
A. Ibuka, A. Taguchi, M. Ishiguro, S. Fushinobu, Y. Ishii, S. Kamitori, K. Okuyama, K. Yamaguchi, M. Konno, and H. Matsuzawa
J. Mol. Biol. 285, 2079-2087 (1999)Zinc and an N-terminal extra stretch of the ferredoxin from a thermoacidophilic archaeon stabilize the molecule at high temperature
K. Kojoh, H. Matsuzawa, and T. Wakagi
Eur. J. Biochem. 264 (1), 85-91 (1999)High level expression of Thermococcus litoralis 4-alpha- glucanotransferase in a soluble form in Escherichia coli with a novel expression system involving minor arginine tRNAs and GroELS
H. Imamura, B. S. Jeon, T. Wakagi, and H. Matsuzawa
FEBS Lett. 457 (3), 393-396 (1999)Purification and properties of the membrane-bound NADH oxidase of a facultatively anaerobic alkaliphile
T. Hamada, T. Wakagi, H. Shiba, and N. Koyama
Arch. Microbiol. 171 (4), 237-242 (1999)Second transmembrane segment of FtsH plays a role in its proteolytic activity and homo-oligomerization
S. Makino, T. Makino, K. Abe, J. Hashimoto, T. Tatsuta, M. Kitagawa, H. Mori, T. Ogura, T. Fujii, S. Fushinobu, T. Wakagi, and H. Matsuzawa
FEBS Lett. 460, 554-558 (1999)
FEBS Lett. 467 (2-3), 365 (2000) [Erratum]Lanthanum binding to aqualysin I, a thermostable serine protease, as probed by lanthanum-139 nuclear magnetic resonance spectrometry
S.-J. Lin, T. Wakagi, H. Matsuzawa, and E. Yoshimura
J. Inorganic Biochem. 77 (3-4), 205-208 (1999)Balanced biosynthesis of major membrane components through regulated degradation of the committed enzyme of lipid A biosynthesis by the AAA protease FtsH (HflB) in Escherichia coli
T. Ogura, K. Inoue, T. Tatsuta, T. Suzaki, K. Karata, K. Young, L. H. Su, C. A. Fierke, J. E. Jackman, C. R. H. Raetz, J. Coleman, T. Tomoyasu, and H. Matsuzawa
Mol. Microbiol. 31 (3), 833-844 (1999)Substrate specificity of aqualysin I altered by an organic solvent, DMSO
T. Tanaka, H. Matsuzawa, and T. Ohta
Biosci. Biotech. Biochem. 63 (2), 446-448 (1999)Properties of a subtilisin-like proteinase from a psychrotrophic Vibrio species - Comparison with proteinase K and aqualysin I
M. M. Kristjansson, O. T. Magnusson, H. M. Gudmundsson, G. A. Alfredsson, and H. Matsuzawa
Eur. J. Biochem. 260 (3), 752-760 (1999)Identification and designing of the S3 site of aqualysin I, a thermophilic subtilisin-related serine protease
T. Tanaka, H. Matsuzawa, and T. Ohta
J. Biochem. (Tokyo) 125 (6), 1016-1021 (1999)Weakly bound calcium ions involved in the thermostability of aqualysin I, a heat-stable subtilisin-type protease of Thermus aquaticus YT-1
S.-J. Lin, E. Yoshimura, H. Sakai, T. Wakagi, and H. Matsuzawa
Biochim. Biophys. Acta 1433 (1-2), 132-138 (1999)
構造亜鉛研究の進展
若木高善
生化学 71 (1), 69-74 (1999)
Recent progress on the study of structural zinc
T. Wakagi
Seikagaku 71 (1), 69-74 (1999) [Japanese]白麹菌キシラナーゼのＸ線結晶構造解析と好酸性・耐酸性機構
伏信 進矢、松澤 洋
日本醸造協会誌 94 (8), 610-614 (1999)
X-ray crystallography of a xylanase from Aspergillus kawachii and the mechanism of its extremely low pH optimum and acid stability
S. Fushinobu, and H. Matsuzawa
J. Brewing Soc Japan 94 (8), 610-614 (1999) [Japanese]