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2004年度日本農芸化学会 学会賞
微生物の新規窒素代謝の発見とその解明
酵素学研究室教授 祥雲 弘文
The Japan Bioscience, Biotechnology and Agrochemistry Society Award, 2004
Discovery and Elucidation of Novel Microbial Metabolism
Prof. Hirofumi Shoun7th International Symposium on Cytochrome P450 Biodiversity and Biotechnology, Poster Award
Awaji-Yumebutai, Hyogo, Japan, August 1-5 2004
Crystal Structure of Cytochrome P450nor in Complex with an NADH Analogue
Ms. Rieko Oshima
Cloning and enhanced expression of the cytochrome P450nor gene (nicA; CYP55A5) encoding nitric oxide reductase from Aspergillus oryzae
M. Kaya, K. Matsumura, K. Higashida, Y. Hata, A. Kawato, Y. Abe, O. Akita, N. Takaya, and H. Shoun
Biosci. Biotechnol. Biochem. 68 (10), 2040-2049 (2004)Structural evidence for direct hydride transfer from NADH to cytochrome P450nor
R. Oshima, S. Fushinobu, F. Su, L. Zhang, N. Takaya, and H. Shoun
J. Mol. Biol. 342 (1), 207-217 (2004)D88A mutant of cytochrome P450nor provides kinetic evidence for direct complex formation with electron donor NADH
M. Umemura, F. Su, N. Takaya, Y. Shiro, and H. Shoun
Eur. J. Biochem. 271 (14), 2887-2894 (2004)Involvement of a Glu71-Arg64 couple in the access channel for NADH in cytochrome P450nor
F. Su, S. Fushinobu, N. Takaya, and H. Shoun
Biosci. Biotechnol. Biochem. 68 (5), 1156-1159 (2004)Isolation of flavohemoglobin from the Actinomycete Streptomyces antibioticus grown without external nitric oxide stress
Y. Sasaki, N. Takaya, A. Nakamura, and H. Shoun
Biosci. Biotechnol. Biochem. 68 (5), 1106-1112 (2004)Unusual transcription regulation of the niaD gene under anaerobic conditions supporting fungal ammonia fermentation
K. Takasaki, H. Shoun, A. Nakamura, T. Hoshino, and N. Takaya
Biosci Biotechnol Biochem. 68 (4), 978-980 (2004)Nitrous oxide-forming codenitrification catalyzed by cytochrome P450nor
F. Su, N. Takaya, and H. Shoun
Biosci. Biotechnol. Biochem. 68 (2), 473-475 (2004)Fungal ammonia fermentation - A novel metabolic mechanism that couples the dissimilatory and assimilatory pathways of both nitrate and ethanol - Role of acetyl CoA synthetase in anaerobic ATP synthesis
K. Takasaki, H. Shoun, M. Yamaguchi, K. Takeo, A. Nakamura, T. Hoshino, and N. Takaya
J. Biol. Chem. 279 (13), 12414-12420 (2004)Transglycosylation activity of Dictyoglomus thermophilum amylase A
M. Nakajima, H. Imamura, H. Shoun, S. Horinouchi, and T. Wakagi
Biosci. Biotech. Biochem. 68 (11), 2369-2373 (2004)Crystal structure of isocitrate dehydrogenase from Aeropyrum pernix
J.-J. Jeong, T. Sonoda, S. Fushinobu, H. Shoun, and T. Wakagi
PROTEINS: Struct. Funct. Bioinform. 55 (4), 1087-1089 (2004)The first crystal structure of the novel class of fructose-1,6-bisphosphatase present in thermophilic archaea
H. Nishimasu, S. Fushinobu, H. Shoun, and T. Wakagi
Structure 12 (6), 949-959 (2004)Molecular evolution of the ATPase subunit of three archaeal sugar ABC transporters
H. Imamura, B.-S. Jeon, and T. Wakagi
Biochem. Biophys. Res. Comm. 319 (1), 230-234 (2004)Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding
A. Miyanaga, S. Fushinobu, K. Ito, H. Shoun, and T. Wakagi
Eur. J. Biochem. 271 (2), 429-438 (2004)Crystal structure of a family 54 α-L-arabinofuranosidase reveals a novel carbohydrate-binding module that can bind arabinose
A. Miyanaga, T. Koseki, H. Matsuzawa, T. Wakagi, H. Shoun, and S. Fushinobu
J. Biol. Chem. 279 (43), 44907-44914 (2004)Crystallization and preliminary X-ray analysis of cellobiose phosphorylase from Cellvibrio gilvus
M. Hidaka, M. Kitaoka, K. Hayashi, T. Wakagi, H. Shoun, and S. Fushinobu
Acta Cryst. D60 (10), 1877-1878 (2004)Expression, purification, crystallization and preliminary X-ray analysis of α-L-arabinofuranosidase B from Aspergillus kawachii
A. Miyanaga, T. Koseki, H. Matsuzawa, T. Wakagi, H. Shoun, and S. Fushinobu
Acta Cryst. D60 (7), 1286-1288 (2004)Chitobiose phosphorylase from Vibrio proteolyticus, a member of glycosyl transferase family 36, has a clan GH-L-like (α/α)6 barrel fold
M. Hidaka, Y. Honda, M. Kitaoka, S. Nirasawa, K. Hayashi, T. Wakagi, H. Shoun, and S. Fushinobu
Structure 12 (6), 937-947 (2004)Escherichia coli cyclophilin B binds a highly distorted form of trans-prolyl peptide isomer
M. Konno, Y. Sano, K. Okudaira, Y. Kawaguchi, Y. Yamagishi-Ohmori, S. Fushinobu, and H. Matsuzawa
Eur. J. Biochem. 271 (18), 3794-3803 (2004)Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium
M. Nishimoto, S. Fushinobu, A. Miyanaga, T. Wakagi, H. Shoun, K. Sakka, K. Ohmiya, S. Nirasawa, M. Kitaoka, and K. Hayashi
Acta Cryst. D60 (2), 342-343 (2004)
微生物の新規窒素代謝の発見とその解明
（2004年度日本農芸化学会学会賞 受賞総説）
祥雲弘文
日本農芸化学会誌 78 (8), 724-429 (2004)
Novel dissimilatory nitrogen metabolisms by microorganisms
H. Shoun
(Award Review of The Japan Bioscience, Biotechnology and Agrochemistry Society Award, 2004)
Nippon Nogeikagaku Kaishi-Journal of the Japan Society for Bioscience Biotechnology and Agrochemistry 78 (8), 724-429 (2004)Anaerobic respiration in the fungal mitochondrion and cytochrome P450nor
H. Shoun, N. Takaya, and S. Fushinobu
(Meeting Abstract of the 3rd International Conference on the Biology, Chemistry and Therapeutic Applications of Nitric Oxide. 4th Annual Scientific Meeting of the Nitric Oxide Society of Japan)
Nitric Oxide - Biology and Chemistry 11 (1): 36-37 (2004)Cellulase and hemicellulase genes of white koji mold (Aspergillus kawachii)
K. Ito, H. Shimoi, K. Iwashita, and S. Fushinobu
In Biotechnology of Lignocellulose Degradation and Biomass Utilization (Proceedings of MIE BIOFORUM 2003; Ohmiya, K., Sakka, K., Karita, S., Kimura, T., Sakka, M. and Onishi, Y., eds), pp. 521-527, Uni Publishers, Tokyo, Japan
祥雲 弘文
「シトクロムP４５０：微生物P４５０を中心に」
かずさBTフォーラム2004 第1回「水酸化酵素の探索研究〜新たな展開へ」
招待講演 2004年7月13日
千葉県木更津市 かずさアーク かずさアカデミアホール
13 July, 2004, Chiba , Japan祥雲 弘文
「微生物の新規窒素代謝の発見とその解明」
日本農芸化学会創立８０周年記念 東北・関東支部合同支部会
受賞記念講演・シンポジウム 2004年7月3日
東北大学農学部
3 July, 2004, Sendai, Japan祥雲 弘文
「微生物の新規窒素代謝の発見とその解明」
2004年度日本農芸化学会大会（広島）
2004年度日本農芸化学会賞受賞記念講演 2004年3月28日
広島国際会議場
28-31 March, 2004, Hiroshima, Japan祥雲 弘文
「地上の窒素循環に関わる微生物窒素代謝とP450nor」
日本薬学会第１２４年会（大阪） 特別講演 2004年3月30日
コスモスクエア国際交流センター
29 - 30 March, 2004 , Osaka , Japan
Aerobic denitrifying bacteria that produce low levels of nitrous oxide
N. Takaya, M. A. B. Catalan-Sakairi, Y. Sakaguchi, I. Kato, Z. Zhou, and H. Shoun
Appl. Environ. Microbiol. 69 (6), 3152-3157 (2003)Hybrid respiration in the denitrifying mitochondria of Fusarium oxysporum
N. Takaya, S. Kuwazaki, Y. Adachi, S. Suzuki, T. Kikuchi, H. Nakamura, Y. Shiro, and H. Shoun
J. Biochem. 133 (4), 461-465 (2003)A possible role of NADPH-dependent cytochrome P450nor isozyme in glycolysis under denitrifying conditions
T. Watsuji, N. Takaya, A. Nakamura, and H. Shoun
Biosci. Biotech. Biochem. 67 (5), 1109-1114 (2003)Denitrification of nitrate by the fungus Cylindrocarpon tonkinense
T. Watsuji, N. Takaya, A. Nakamura, and H. Shoun
Biosci. Biotech. Biochem. 67 (5), 1115-1120 (2003)Formate-forming fungal catabolic pathway to supply electrons to nitrate respiration
S. Kuwazaki, N. Takaya, A. Nakamura, and H. Shoun
Biosci. Biotech. Biochem. 67 (4), 937-939 (2003)The crystal structure of sulerythrin, a rubrerythrin-like protein from a strictly aerobic archaeon, Sulfolobus tokodaii strain 7, shows unexpected domain swapping
S. Fushinobu, H. Shoun, and T. Wakagi
Biochemistry, 42 (40), 11707-11715 (2003)Crystal structure of an ADP-dependent glucokinase from Pyrococcus furiosus: Implications for a sugar-induced conformational change in ADP-dependent kinase
S. Ito, S. Fushinobu, J.-J. Jeong, I. Yoshioka, S. Koga, H. Shoun, and T. Wakagi
J. Mol. Biol. 331 (4), 871-883 (2003)Archaeal ADP-dependent phosphofructokinase: expression, purification, crystallization and preliminary crystallographic analysis
J.-J. Jeong, S. Fushinobu, S. Ito, H. Shoun, and T. Wakagi
Acta Cryst. D59 (7), 1327-1329 (2003)Unique metal dependency of cytosolic alpha-mannosidase from Thermotoga maritima, a hyperthermophilic bacterium.
M. Nakajima, H. Imamura, H. Shoun, and T. Wakagi
Arch. Biochem. Biophys. 415 (1), 87-93 (2003)Characterization of the cupin-type phosphoglucose isomerase from the hyperthermophilic archaeon Thermococcus litoralis
J.-J. Jeong, S. Fushinobu, S. Ito, B.-S. Jeon, H. Shoun, and T. Wakagi
FEBS Lett. 535 (1-3), 200-204 (2003)Sulerythrin, the smallest member of the rubrerythrin family, from a strictly aerobic and thermoacidophilic archaeon, Sulfolobus tokodaii strain 7
T. Wakagi
FEMS Microbiol. Lett. 222 (1), 33-37 (2003)Regulation of the aerobic respiratory chain in the facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense
T. Nunoura, Y. Sako, T. Wakagi, and A. Uchida
Microbiology 149 (3), 673-688 (2003)Crystal structures of 4-alpha-glucanotransferase from Thermococcus litoralis and its complex with an inhibitor
H. Imamura, S. Fushinobu, M. Yamamoto, T. Kumasaka, B.-S. Jeon, T. Wakagi, and H. Matsuzawa
J. Biol. Chem. 278 (21), 19378-19386 (2003)Crystal structure of a histidine-tagged serine hydroase involved in the carbazole degradation (CarC enzyme)
H. Habe, K. Morii, S. Fushinobu, J.-W. Nam, Y. Ayabe, T. Yoshida, T. Wakagi, H. Yamane, H. Nojiri, and T. Omori
Biochem. Biophys. Res. Commun. 303 (2), 631-639 (2003)Identification and molecular characterization of a novel type of alpha-galactosidase from Pyrococcus furiosus
J. F. T. van Lieshout, C. H. Verhees, T. J. G. Ettema, S. van der Sar, H. Imamura, H. Matsuzawa, J. van der Oost, and W. M. de Vos
Biocatal. Biotransform. 21 (4/5), 243-252 (2003)
真菌脱窒系の発見とP450norの構造・機能相関解明
祥雲 弘文
バイオサイエンスとインダストリー 61, 11-16 (2003)カビのアンモニア発酵：土壌真菌は通気条件の変化に応じてエネルギー代謝を変える通性嫌気性生物
周 哲敏、祥雲 弘文
化学と生物 41, 561-562 (2003)P４５０の分子生物学 講談社サイエンティフィック (ISBN 4-06-153677-X)
祥雲 弘文 共著
6.2, 6.3 218 - 233 (2003)糖質加水分解酵素のバレル構造：ファミリー42と57のX線結晶構造解析
伏信 進矢、日高 將文、今村 博臣
構造生物 8 (3), 6-15 (2003)
Barrel structures of glycosyl hydrolases: crystal structures of family 42 and 57 enzymes
S. Fushinobu, M. Hidaka, and H. Imamura
J. Struct. Biol. Sakabe Project 8 (3), 6-15 (2003) [Japanese]Crystal structures of ADP-dependent glucokinases in novel glycolytic pathway of hyperthermophilic archaea
S. Ito, J.-J. Jeong, S. Fushinobu, and T. Wakagi
Photon Factory Activity Report #21 2003 Part A, Highlights [Biological Science], pp. 38-39
Hirofumi Shoun
Structure of cytochrome P450nor in complex with an NADH
12th Japanese-German Workshop on Enzyme Technology "Invited Speaker"
16-18 November, 2003, Shiga, Japan祥雲 弘文
「ミトコンドリア嫌気呼吸とＰ４５０」
酵素工学研究会第50回講演会
24 October, 2003, University of Tokyo, Japan祥雲 弘文
第1回 財団法人農学会 公開セミナー
「未利用バイオマスをいかに活用するか」ー農業と市民生活における有機性廃棄物の循環利用ーにおけるコメンテーター
6 September, 2003, University of Tokyo, JapanHirofumi Shoun
Direct electron transfer from NADH to cytochrome P450nor
The 9th Southeast Asian-Western Pacific Regional Meeting of Pharmacologists "Invited Speaker"
19-23 August, Busan, KOREA祥雲 弘文
「微生物の新規窒素代謝とＰ４５０」
神戸大学 学術講演会
25-26 July, 2003, Koube, Japan祥雲 弘文
「窒素サイクルに関わる微生物の新規窒素代謝」
平成15年度 発酵と代謝研究会シンポジウム 微生物新機能を求めて
(財)バイオインダストリー協会 発酵と代謝研究会 主催
11 July, 2003, Tokyo, Japan祥雲 弘文
「地球温暖化抑止にバイオテクノロジーはいかに貢献できるか - 微生物の新規窒素呼吸系 -」
第12回 TIARA研究発表会 特別講演
19-20 June, 2003, Takasaki, Japan
2002年度有馬啓記念バイオインダストリー協会賞
真菌脱窒系の発見とP450norの構造・機能相関解明
酵素学研究室教授 祥雲 弘文
Professor Kei Arima Memorial Award, Japan Bioindustry Association, 2002
Dicovery of the Fungal Denitrifying System and Studies on Structure-Function Relationship of P450nor
Prof. Hirofumi Shoun
Genetic engineering using fungal flavohemoglobin for constructing Pseudomonas stutzeri strain emitting less nitrous oxide
N. Takaya, H. Shoun
J. Biosci. Bioeng. 94: (3), 282-284 (2002)The B' helix determines cytochrome P450nor specificity for the electron donors NADH and NADPH
L. Zhang, T. Kudo, N. Takaya, and H. Shoun
J. Biol. Chem. 277 (37), 33842-33847 (2002)Ammonia fermentation, a novel anoxic metabolism of nitrate by fungi
Z. Zhou, N. Takaya, A. Nakamura, M. Yamaguchi, K. Takeo, and H. Shoun
J. Biol. Chem. 277 (3), 1892-1896 (2002)Nitrate reductase-formate dehydrogenase couple involved in the fungal denitrification by Fusarium oxysporum
H. Uchimura, H. Enjoji, T. Seki, A. Taguchi, N. Takaya, and H. Shoun
J. Biochem. 131 (4), 579-586 (2002)Codenitrification and denitrification are dual metabolic pathways by which dinitrogen evolves from nitrate in Streptomyces antibioticus
Y. Kumon, Y. Sasaki, I. Kato, N. Takaya, H. Shoun, and T. Beppu
J. Bacteriol. 184 (11), 2963-2968 (2002)Kinetic analysis on hydroxylation of saturated fatty acids by recombinant P450foxy produced by Escherichia coli espression system.
T. Kitazume, A. Tanaka, N. Takaya, A. Nakamura, S. Matsuyama, T. Suzuki, and H. Shoun
Eur. J. Biochem. 269 (8), 2075-2082 (2002)Isotope effects and intermediates in the reduction of NO by P450nor.
A. Daiber, T. Nauser, P. Weber, C. Hultschig, N. Takaya, H. Shoun, and V. Ullrich
J. Inorg. Biochem. 88 (3-4), 343-352 (2002)Transcriptional control of nitric oxide reductase gene (CYP55) in the fungal denitrifier Fusarium oxysporum
N. Takaya, H. Uchimura, and H. Shoun
Biosci. Biotech. Biochem. 66 (5), 1039-1045 (2002)Well maintained CYP genes in sandwich-culturing hepatocytes: quantitative analysis using real-time PCR method
N. Toritsuka, Y. Nonogaki, Y. Hori, S. Sawada, H. Shoun, and S. Motooka
Res. Commun. Mol. Pathol. Pharmacol. (2002)Crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with cleavage products
S. Fushinobu, T. Saku, M. Hidaka, S.-Y. Jun, H. Nojiri, H. Yamane, H. Shoun, T. Omori, and T. Wakagi
Protein Science 11(9), 2184-2195 (2002)Advantages of sandwich-culturing hepatocytes for assessment of chronic hepatotoxicity.
N. Toritsuka, Y. Nonogaki, Y. Hori, T. Sugihara, S. Sawada, H. Shoun, and S. Motooka
Res. Commun. Pharmacol. Toxicol. (2002)Trimeric crystal structure of the glycoside hydrolase family-42 beta-galactosidase from Thermus thermophilus A4 and the Structure of its Complex with Galactose
M. Hidaka, S. Fushinobu, N. Ohtsu, H. Motoshima, H. Matsuzawa, H. Shoun, and T. Wakagi
J. Mol. Biol. 322 (1), 79-91 (2002)Aeropyrum pernix K1, a strictly aerobic and hyperthermophilic archaeon, has two terminal oxidases, cytochrome ba3 and cytochrome aa3
R. Ishikawa, Y. Ishido, A. Tachikawa, H. Kawasaki, H. Matsuzawa, and T. Wakagi
Arch. Microbiol. 179 (1), 42-49 (2002)CYP119 plus a Sultolobus tokodaii strain 7 ferredoxin and 2-oxoacid : ferredoxin oxidoreductase constitute a high-temperature cytochrome P450 catalytic system
A. V. Puchkaev, T. Wakagi, and P. R. O. de Montellano
J. Am. Chem. Soc. 124 (43), 12682-12683 (2002)Sodium-23 and lanthanum-139 nuclear magnetic resonance studies of cation binding to aqualysin I, a thermostable serine protease
K. Suefuji, S.-J. Lin, T. Wakagi, H. Matsuzawa, and E. Yoshimura
Biosci. Biotech. BIochem. 66 (6), 1281-1286 (2002)Substrate recognition by 2-oxoacid : ferredoxin oxidoreductase from Sulfolobus sp strain 7
E. Fukuda, and T. Wakagi
Biochim. Biophys. Acta 1597 (1), 74-80 (2002)Acyl-intermediate structures of the extended-spectrum class A beta -lactamase, Toho-1, in complex with cefotaxime, cephalothin and benzylpenicillin
T. Shimamura, A. Ibuka, S. Fushinobu, T. Wakagi, M. Ishiguro, Y. Ishii, and H. Matsuzawa
J. Biol. Chem. 277 (48), 46601-46608 (2002)Purification, characterization, and steady-state kinetics of a meta-cleavage compound hydrolase from Pseudomonas fluorescens IP01
T. Saku, S. Fushinobu, S. Y. Jun, N. Ikeda, H. Nojiri, H. Yamane, T. Omori, and T. Wakagi
J. Biosci. Bioeng. 93 (6), 568-574 (2002)Crystal structure of the liganded anti-gibberellin A4 antibody 4-B8(8)/E9 Fab fragment
T. Murata, S. Fushinobu, M. Nakajima, O. Asami, T. Sassa, T. Wakagi, and I. Yamaguchi
Biochem. Biophys. Res. Comm. 293 (1), 489-496 (2002)Zinc-coordination of aspartic acid-76 in Sulfolobus ferredoxin is not required for thermal stability of the molecule
K. Kojoh, E. Fukuda, H. Matsuzawa, and T. Wakagi
J. Inorg. Biochem. 89:(1-2), 69-73 (2002)A novel bifunctional molybdo-enzyme catalyzing both decarboxylation of indolepyruvate and oxidation of indoleacetaldehyde from a thermoacidophilic archaeon, Sulfolobus sp strain 7
T. Wakagi, E. Fukuda, Y. Ogawa, H. Kino, and H. Matsuzawa
FEBS Lett. 510 (3), 196-200 (2002)An absolute requirement of fructose 1,6-bisphosphate for the Lactobacillus casei L-lactate dehydrogenase activity induced by a single amino acid substitution
K. Arai, A. Hishida, M. Ishiyama, T. Kamata, H. Uchikoba, S. Fushinobu, H. Matsuzawa, and H. Taguchi
Protein Engineering 15 (1), 35-41 (2002)Crystal structure of non-allosteric L-lactate dehydrogenase from Lactobacillus pentosus at 2.3 angstrom resolution: Specific interactions at subunit interfaces
H. Uchikoba, S. Fushinobu, T. Wakagi, M. Konno, H. Taguchi, and H. Matsuzawa
Proteins 46 (2), 206-214 (2002)HscA is involved in the dynamics of FtsZ-ring formation in Escherichia coli K12
T. Uehara, H. Matsuzawa, and A. Nishimura
Genes Cells 6 (9), 803-814 (2001)
Genes Cells 6 (11), 1017-1018 (2001) [Erratum]
カビと放線菌の新規窒素呼吸系
祥雲弘文
バイオサイエンスとインダストリー 60 (7), 11-15 (2002)
Novel nitrogen respiration by fungi and actinomycetes
H. Shoun
Bioscience and Industry 60 (7), 11-15 (2002) [Japanese]Thermus thermophilus A4株由来・新規耐熱性βガラクトシダーゼおよび基質複合体のX線結晶構造解析
（糖質関連酵素化学シンポジウム）
日高將文、伏信 進矢、大津奈穂美、元島英雅、松澤洋、祥雲弘文、若木高善
応用糖質科学 49 (2), 175-180 (2002)
X-ray crystallography of a novel thermostable beta-galactosidase from Thermus thermophilus A4, and its complex structure with galactose
(Proceedings of the Symposium on Amylases and Related Enzymes, 2001)
M. Hidaka, S. Fushinobu, N. Ohtsu, H. Motoshima, H. matsuzawa, H. Shoun, and T. Wakagi
J. Appl. Glycosci. 49 (2), 175-180 (2002) [Japanese]Molecular mechanism of nitric oxide reduction catalyzed by fungal nitric oxide reductase.
Obayashi, E., shimizu, H., Park, s.-Y., Nakamura, H., Takahashi, S., Shoun, H., and Shiro, Y
Oxygen and Life: Oxygenases, Oxidases and Lipid Mediators; Excerpta Medica International Congress Series 1233 (Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F., eds) pp. 59-62, Elsevier, (2002)Cytochromes P450nor and P450foxy of the fungus Fusarium oxysporum
Shoun, H., and Takaya, N.
Oxygen and Life: Oxygenases, Oxidases and Lipid Mediators; Excerpta Medica International Congress Series 1233 (Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F., eds) pp. 89-97, Elsevier, (2002)Analysis of fungal P450foxy (CYP505), a fused protein of cytochrome P450 and its reductase
Kitazume, T., Tanaka, A., Matsuyama, S., Takaya, N., and Shoun, H.
Oxygen and Life: Oxygenases, Oxidases and Lipid Mediators; Excerpta Medica International Congress Series 1233 (Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F., eds) pp. 143-149, Elsevier, (2002)Distribution, structure and function of fungal nitric oxide reductase P450nor - recent advances.
Zhang, L., Kudo, T., Takaya, N., and Shoun, H.
Oxygen and Life: Oxygenases, Oxidases and Lipid Mediators; Excerpta Medica International Congress Series 1233 (Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F., eds) pp. 89-97, Elsevier, (2002)Nitric Oxide synthesis by the Actinomycete S.antibioticus
Yasuyuki Sasaki, Naoki Takaya, Akira Nakamura, Hirofumi Shoun
Arch. Biochem. Biophys., Part B : NITRIC OXIDE, Biology and Chemistry 6, 459-460(2002)Binding of NAD(P)H to NO reductase Cytochrome P450nor
Hirofumi Shoun, Li Zhang, Takashi Kudo, Fei Su, Naoki Tkaya
Arch. Biochem. Biophys., Part B : NITRIC OXIDE, Biology and Chemistry 6, 463-463(2002)Fungal cytochrome P450nor and flavohemoglobin are involved in survival under NO stress
Naoki Takaya, Hidetoshi Morita, Hirofumi Shoun
Arch. Biochem. Biophys., Part B : NITRIC OXIDE, Biology and Chemistry 6, 472-472(2002)Recognition of tRNA by aminoacyl-tRNA synthetase from hyperthermophilic archaea, Aeropyrum pernix K1.
J. Yokozawa, Y. Nagaoka, T. Umehara, J. Iwaki, Y. Kawarabayasi, Y. Koyama, Y. Sako, T. Wakagi, A. Kuno, and T. Hasegawa
Nucleic Acids Res. Suppl. (1), 117-118 (2002)Molecular recognition of threonine tRNA by threonyl-tRNA synthetase from an extreme thermophilic archaeon, Aeropyrum pernix K1
Y. Nagaoka, J. Yokozawa, T. Umehara, J. Iwaki, K. Okamoto, Y. Kawarabayasi, Y. Koyama, Y. Sako, T. Wakagi, A. Kuno, and T. Hasegawa
Nucleic Acids Res. Suppl. (2), 81-82 (2002)
祥雲 弘文
｢カビ Fusarium oxysporum の P450nor と P450foxy」
1st Workshop on P450 Biodiversity and Biotechnology
29-30 November 2002, Awaji-Yumebutai, Hyogo, Japan祥雲 弘文
「亜酸化窒素（N20)抑止型好気脱窒システムの構築と水処理への応用」
主催：JST 科学技術振興事業団 Japan Science and Technology Corporation
26 November, 2002, Tokyo, Japan祥雲 弘文
真菌脱窒系の発見とP450norの構造・機能相関説明
財団法人化学技術戦略推進機構 (JCII) にて
20 November 2002, Tokyo, Japan祥雲 弘文
｢真菌脱窒系の発見とP450norの構造・機能相関解明
バイオインダストリー協会賞受賞記念講演
24 October 2002, Tokyo, JapanHirofumi Shoun
Application of p450nor and P450foxy to Green Chemistry
Korea-Japan／Japan-Korea Biotechnology Industry Investment & Cooperation Conference ＜韓日 生物産業交流協力地行＞
30 September - 2 October 2002, Seoul, KoreaHirofumi Shoun
Diversified functions and application of cytochrome P450
10th Anniversary Novozymes Japan Enzyme Symposium
27 September 2002, Kyoto, Japan祥雲 弘文
特別講演「地球環境と微生物バイオテクノロジー」
金沢大学 集中講義「微生物を利用したバイオテクノロジー」
17-18 September 2002, Kanazawa, JapanHirofumi Shoun
2nd International Conference, Biology,Chemistry and Therapeutic Applications of Nitric Oxide (NO 2002)
＜第２回国際NO会議>
16-20 June 2002, Prague, Czech Republic