1996-1998年の研究業績

Publications & Activities from 1996 to 1998


Present-2013 2012-2008 2007-2005 2004-2002 2001-1999

1998 1997 1996

1995-1993


1998

Original Papers

Cloning of the gene for inorganic pyrophosphatase from a thermoacidophilic archaeon, Sulfolobus sp. strain 7, and overproduction of the enzyme by coexpression of tRNA for Arginine rare codon
Wakagi-T; Oshima-T; Imamura-H; Matsuzawa
Biosci. Biotech. Biochem. 62 (12), 2408-2414 (1998)

Homotropic activation via the subunit interaction and allosteric symmetry revealed on analysis of hybrid enzymes of L-lactate dehydrogenase
S. Fushinobu, T. Ohta, and H. Matsuzawa
J. Biol. Chem. 273 (5), 2971-2976 (1998)

Crystallographic and mutational analyses of an extremely acidophilic and acid-stable xylanase: biased distribution of acidic residues and importance of Asp37 for the catalysis at low pH
S. Fushinobu, K. Ito, M. Konno T. Wakagi, and H. Matsuzawa
Protein Engineering, 11 (12), 1121-1128 (1998)

Conversion of neopullulanase-alpha-amylase from Thermoactinomyces vulgaris R-47 into an amylopullulanase-type enzyme
A. Ibuka, T. Tonozuka, H. Matsuzawa, and H. Sakai
J. Biochem. (Tokyo) 123 (2), 275-282 (1998)

Cloning and sequencing of the gene encoding Toho-2, a class A beta-lactamase preferentially inhibited by tazobactam
L. Ma, Y. Ishii, M. Ishiguro, H. Matsuzawa, and K. Yamaguchi
Antimicrob. Agents Chemotherapy 42 (5), 1181-1186 (1998)

ftsE(Ts) affects translocation of K+-pump proteins into the cytoplasmic membrane of Escherichia coli
H. Ukai, H. Matsuzawa, K. Ito, M. Yamada, A. Nishimura
J. Bacteriol. 180 (14), 3663-3670 (1998)

Thermostable beta-galactosidase from an extreme thermophile, Thermus sp. A4: Enzyme purification and characterization, and gene cloning and sequencing
N. Ohtsu, H. Motoshima, K. Goto, F. Tsukasaki, and H. Matsuzawa
Biosci. Biotech. Biochem. 62 (8), 1539-1545 (1998)

Stability of thermostable enzyme, aqualysin I; a subtilisin-type serine protease from Thermus aquaticus YT-1
T. Tanaka, H. Matsuzawa, and H. Ohta
Biosci. Biotech. Biochem. 62 (9), 1806-1808 (1998)

P1-specificity of aqualysin I (a subtilisin-type serine protease) from Thermus aquaticus YT-1, using P1-substituted derivatives of Streptomyces subtilisin inhibitor
T. Tanaka, H. Matsuzawa, S. Kojima, I. Kumagai, K. Miura, and T. Ohta
Biosci. Biotech. Biochem. 62 (10), 2035-2038 (1998)

Purification and characterization of a feruloylesterase from Aspergillus awamori
T. Koseki, S. Furuse, K. Iwano, H. Matsuzawa
Biosci. Biotech. Biochem. 62 (10), 2032-2034 (1998)

Substrate specificity of aqualysin I, a bacterial thermophilic alkaline serine protease from Thermus aquaticus YT-1: Comparison with proteinase K, subtilisin BPN ' and subtilisin Carlsberg
T. Tanaka, H. Matsuzawa, and T. Ohta
Biosci. Biotech. Biochem. 62 (11), 2161-2165 (1998)

Engineering of S2 site of aqualysin I; Alteration of P2 specificity by excluding P2 side chain
T. Tanaka, H. Matsuzawa, and T. Ohta
Biochemistry 37 (50), 17402-17407 (1998)

Presence of Na+-stimulated V-type ATPase in the membrane of a facultatively anaerobic and halophilic alkaliphile
Kaieda-N; Wakagi-T; Koyama-N
FEMS Microbiol. Lett. 167 (1), 57-61 (1998)

Others

蛋白質の安くて速い濃縮法
若木高善
実験医学 16 (17) 2250-2251 (1998)

Aspergillus kawachii 酸性キシラナーゼCのX線結晶構造解析
(糖質関連酵素化学シンポジウム)
伏信 進矢、伊藤 清、今野 美智子、松沢 洋
応用糖質科学 45 (2), 139-145 (1998)
X-ray crystallograpy of acid xylanase from Aspergillus kawachii
(Proceedings of the Symposium on Amylases and Related Enzymes, 1997)
S. Fushinobu, K. Ito, M. Konno, and H. Matsuzawa
J. Appl. Glycosci. 45 (2), 139-145 (1998) [Japanese]

乳酸菌LDHの活性調節
伏信 進矢、松沢 洋
バイオサイエンスとインダストリー 56 (10), 685-686 (1998)
Allosteric regulation of lactate dehydrogenase from lactic acid bacteria
S. Fushinobu, and H. Matsuzawa
Bioscience & Industry 56 (10), 685-686 (1998) [Japanese]


1997

Original Papers

An Aspergillus awamori acetylesterase: purification of the enzyme, and cloning and sequencing of the gene
T. Koseki,S. Furuse, K. Iwano, H. Sakai, and H. Matsuzawa
Biochem. J. 326 (2), 485-490 (1997)

4-alpha-glucanotransferase from the hyperthermophilic archaeon Thermococcus litoralis: Enzyme purification and characterization, and gene cloning, sequencing and expression in Escherichia coli
B.-S. Jeon,H. Taguchi,H. Sakai, T. Ohshima, T. Wakagi, and H. Matsuzawa
Eur. J. Biochem. 248, 171-178 (1997)

A silent mutation in the ftsH gene of Escherichia coli that affects FtsH protein production and colicin tolerance
S. Makino, J.-N. Qu, K. Uemori, H. Ichikawa, T. Ogura, and H. Matsuzawa
Mol. Gen. Genet. 254, 578-583 (1997)

Increase of the Protease Activity of Aqualysin I, a Thermostable Serine Protease, by Replacing Asn219 near the Catalytic Residue Ser222
Shie-Jea Lin, Dong-Wook Kim, Yoko Ryugo, Takayoshi Wakagi, and Hiroshi Matsuzawa
Biosci. Biotech. Biochem. 61 (4), 718-719 (1997)

A Carboxy-Terminal Pro-Sequence of Aqualysin I Prevents Proper Folding of the Protease Domain on Its Secretion by Saccharomyces cerevisiae
Dong-Wook Kim, Shie-Jea Lin, Shuji Morita, Ichiro Terada, and Hiroshi Matsuzawa
Biochem. Biophys. Res. Comm. 231, 535-539 (1997)

The crystal structure of zinc-containing ferredoxin from the thermoacidophilic archaeon, Sulfolobus sp. strain 7
Tomomi Fujii, Yasuo Hata, Masato Oozeki, Hideaki Moriyama, Takayoshi Wakagi, Nobuo Tanaka, and Tairo Oshima
Biochemistry 36 (6), 1505-1513 (1997)

Molecular and phylogenetic characterization of isopropylmalate dehydrogenase of a thermoacidophilic archaeon, Sulfolobus sp. srain 7
Toshiharu Suzuki, Yutaka Inoki, Akihiko Yamagishi, Toshio Iwasaki, Takayoshi Wakagi, and Tairo Oshima
J. Bacteriol. 179, 1174-1179 (1997)

Over-production of Thermus protease in dense culture of Escherichia coli using two carbon sources
K. Tsuji, M. Iijima, H. Matsuzawa, and S. Sakamoto
Biotech. Techniques 11 (6), 395-398 (1997)

Involvement of Glu-264 and Arg-235 in the essential interaction between the catalytic imidazole and substrate for the D-lactate dehydrogenase catalysis
H. Taguchi, T. Ohta, H. Matsuzawa
J. Biochem. (Tokyo) 122 (4), 802-809 (1997)

Role of the COOH-terminal pro-sequence of aqualysin I (a heat-stable serine protease) in its extracellular secretion by Thermus thermophilus
D.-W. Kim, Y.-C. Lee, H. Matsuzawa
FEMS Microbiol. Lett. 157 (1), 39-45 (1997)


1996

Original Papers

Allosteric activation of L-lactate dehydrogenase analysed by hybrid enzymes with effector-sensitive and -insensitive subunits
Shinya Fushinobu, Kenji Kamata, So Iwata, Hiroshi Sakai, Takahisa Ohta, and Hiroshi Matsuzawa
J. Biol. Chem. 271 (41) 25611-25616 (1996)

Folding-Dependent in Vitro Protein Splicing of the Saccharomyces cerevisiae VMA1 Protozyme
Masato Kawasaki, Shin-ichi Makino, Hiroshi Matsuzawa, Yoshinori Satow, Yoshikazu Ohya, and Yasuhiro Anraku
Biochem. Biophys. Res. Comm. 222, 827-832 (1996)

The tolZ Gene of Escherichia coli Is Identified as the ftsH Gene
Jian-Ning Qu, Shin-ichi Makino, Hiroyuki Adachi, Yoshinori Koyama, Yoshinori Akiyama, Koreaki Ito, Toshifumi Tomoyasu, Teru Ogura, and Hiroshi Matsuzawa
J. Bacteriol. 178 (12), 3457-3461 (1996)

The active site of carboxypeptidase Taq posesses the active-site motif His-Glu-X-X-His of zinc-dependent endopeptidases and aminopeptidases
Sang-Hyeon Lee, Hayao Taguchi, Etsuro Yoshimura, Etsuo Minagawa, Shuichi Kaminogawa, Takahisa Ohta, and Hiroshi Matsuzawa
Protein Engineering 9 (6), 467-469 (1996)

Efficient production of Thermus protease aqualysin I in Escherichia coli: effects of cloned gene structure and two-stage culture
S. Sakamoto, I. Terada, Y.-C. Lee, K. Uehara, H. Matsuzawa, M. Iijima
Appl. Microbiol. Biotechnol. 45, 94-101 (1996)

Comparison of Transglycosylation Catalyzed by Two Alpha-Amylases from Thermoactinomyces vulgaris R-47
Takashi Tonozuka, Akiko Ibuka, Hiroshi Sakai, Hiroshi Matsuzawa, and Yoshiyuki Sakano
J. Appl. Glycosci. 43 (1), 95-98 (1996)

A 38 kDa precursor protein of aqualysin I (a thermophilic subtilisin-type protease) with a C-terminal extended sequence: its purification and in vitro processing
Keisuke Kurosaka, Takahisa Ohta, and Hiroshi Matsuzawa
Mol. Microbiol. 20, 385-389 (1996)

Molecular cloning, sequencing, and heterologous expression of a novel zinc-containing ferredoxin gene from a thermacidophilic archaeon Sulfolobus sp. strain 7
Takayoshi Wakagi, Tomomi Fujii, and Tairo Oshima
Biochem. Biophys. Res. Commun. 225, 489-493 (1996)

Novel zinc-binding ceter in thermoacidophilic archaeal ferredoxins
Tomomi Fujii, Yasuo Hata, Takayoshi Wakagi, Nobuo Tanaka, and Tairo Oshima
Nat. Struct. Biol. 3, 834-837 (1996)

2-Oxoacid:ferredoxin oxidoreductase from the thermoacidophilic archaeon, Sulfolobus sp. strain 7
Qian Zhang, Toshio Iwasaki, Takayoshi Wakagi, and Tairo Oshima
J. Biochem. (Tokyo), 120, 587-599 (1996)


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