2005-2007年の研究業績

Publications & Activities from 2005 to 2007


Present-2013 2012-2008

2007 2006 2005

2004-2002 2001 - 1999 1998 - 1996 1995 - 1993

2007

Awards

平成18年度 東京大学大学院農学生命科学研究科長賞
西増 弘志
Dean's Award from the Graduate School of Agricultural and Life Sciences, The University of Tokyo
Hiroshi Nishimasu

2007年度日本農芸化学会関東支部若手奨励賞(ポスター賞)
ビフィズス菌由来オリゴ糖トランスポーター可溶性サブユニットの立体構造解析
鈴木 龍一郎 他

Original Papers

Characterization of a thermostable carboxylesterase from the hyperthermophilic bacterium Thermotoga maritima
S. Kakugawa, S. Fushinobu, T. Wakagi, and H. Shoun
Appl. Microbiol. Biotechnol. 74 (3), 585-591 (2007)

Crystal structures of an ATP-dependent hexokinase with broad substrate specificity from the hyperthermophilic archaeon Sulfolobus tokodaii
H. Nishimasu, S. Fushinobu, H. Shoun, and T. Wakagi
J. Biol. Chem. 282 (13), 9923-9931 (2007)

Crystal structure of intracellular family 1 β-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium
Y. Nijikken, T. Tsukada, K. Igarashi, M. Samejima, T. Wakagi, H. Shoun, and S. Fushinobu
FEBS Lett. 581 (7), 1514-1520 (2007)

Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217
J. Wada, R. Suzuki, S. Fushinobu, M. Kitaoka, T. Wakagi, H. Shoun, H. Ashida, H. Kumagai, T. Katayama, and K. Yamamoto
Acta Cryst. F63 (9), 751-753 (2007)

Molecular anatomy of the alkaliphilic xylanase from Bacillus halodurans C-125
M. Nishimoto, S. Fushinobu, A. Miyanaga, M. Kitaoka, and K. Hayashi
J. Biochem. 141 (5), 709-717 (2007)

Structural insight into concerted inhibition of α2β2-type aspartate kinase from Corynebacterium glutamicum
A. Yoshida, T. Tomita, T. Kurihara, S. Fushinobu, T. Kuzuyama, and M. Nishiyama
J. Mol. Biol. 368 (2), 521-536 (2007)

Others

超好熱性古細菌の新規なヘキソキナーゼの基質結合に伴う構造変化
西増弘志、伏信進矢、祥雲弘文、若木高善
Photon Factory News 25 (3), 22-25 (2007) [PDF]
A novel hexokinase from hyperthermophilic archaeon and its structural changes on substrate binding
H. Nishimasu, S. Fushinobu, H. Shoun, and T. Wakagi
Photon Factory News 25 (3), 22-25 (2007) [Japanese]

New structural insights on carbohydrate-active enzymes
S. Fushinobu, M. Hidaka, A. Miyanaga and H. Imamura
J. Appl. Glycosci. 54 (2), 95-102 (2007)


2006

Original Papers

Denitrification by the mix-culturing of fungi and bacteria with shell
D. Liu, S. Zhang, Y. Zheng, and H. Shoun
Microbiol Res. 161 (2),132-137 (2006)

Tuberibacillus calidus gen. nov., sp. nov., isolated from a compost pile and reclassification of Bacillus naganoensis Tomimura et al. 1990 as Pullulanibacillus naganoensis gen. nov., comb. nov. and Bacillus laevolacticus Andersch et al. 1994 as Sporolactobacillus laevolacticus comb. nov.
K. Hatayama, H. Shoun, Y. Ueda, and A. Nakamura
Int. J. Syst. Evol. Microbiol. 56 (11), 2545-2551 (2006)

Improving the catalytic efficiency of a meta-cleavage product hydrolase (CumD) from Pseudomonas fluorescens IP01
S.-Y. Jun, S. Fushinobu, H. Nojiri, T. Omori, H. Shoun and T. Wakagi
Biochim. Biophys. Acta - Proteins Proteomics 1764 (7), 1159-1166 (2006)

Identification and characterization of an ATP-Dependent hexokinase with broad substrate specificity from the hyperthermophilic archaeon Sulfolobus tokodaii
H. Nishimasu, S. Fushinobu, H. Shoun, and T. Wakagi
J. Bacteriol. 188 (5), 2014-2019 (2006)

Crystallization and preliminary X-ray analysis of cytosolic α-mannosidase from Thermotoga maritima
M. Nakajima, S. Fushinobu, H. Imamura, H. Shoun, and T. Wakagi
Acta Cryst. F62 (2), 104-105 (2006)

The family 42 carbohydrate-binding module of family 54 α-L-arabinofuranosidase specifically binds the arabinofuranose side chain of hemicellulose
A. Miyanaga, T. Koseki, Y. Miwa, Y. Mese, S. Nakamura, A. Kuno, J. Hirabayashi, H. Matsuzawa, T. Wakagi, H. Shoun and S. Fushinobu
Biochem. J. 399 (3), 503-511 (2006)

Structural dissection of the reaction mechanism of cellobiose phosphorylase
M. Hidaka, M. Kitaoka, K. Hayashi, T. Wakagi, H. Shoun, and S. Fushinobu
Biochem. J. 398 (1), 37-43 (2006)

N-Linked oligosaccharides of Aspergillus awamori feruloyl esterase are important for thermostability and catalysis
T. Koseki, K. Takahashi, T. Handa, Y. Yamane, S. Fushinobu, K. Hashizume
Biosci. Biotechnol. Biochem. 70 (10), 2476-2480 (2006)

Mutational analysis of N-glycosylation recognition sites on the biochemical properties of Aspergillus kawachii α-L-arabinofuranosidase 54
T. Koseki, Y. Miwa, Y. Mese, A. Miyanaga, S. Fushinobu, T. Wakagi, H. Shoun, H. Matsuzawa, and K. Hashizume
Biochim. Biophys. Acta - Gen. Subjects 1760 (9), 1458-1464 (2006)

Structural basis for the alteration of coenzyme specificity in a malate dehydrogenase mutant
T. Tomita, S. Fushinobu, T. Kuzuyama and M. Nishiyama
Biochem. Biophys. Res. Commun. 347 (2), 502-508 (2006)

Others

ミトコンドリア嫌気呼吸とカビの通性嫌気性
祥雲弘文
蛋白質 核酸 酵素 51 (5), 419-429 (2006)
Denitrification and anaerobic energy producing mechanisms by fungi
H. Shoun
Tanpakushitsu Kakusan Koso 51 (5), 419-429 (2006) [Japanese]

Crystal structure of GH54 α-L-arabinofuranosidase and unique function of CBM42 attached to it
A. Miyanaga, T. Koseki, H. Matsuzawa, T. Wakagi, H. Shoun and S. Fushinobu
(Proceedings of the Symposium on Amylase and Related Enzymes, 2005)
J. Appl. Glycosci. 53 (2), 143-148 (2006)

Lectures etc.

祥雲 弘文
「微生物による窒素循環と地球環境」
東京大学の生命科学シンポジウム
15 April 2006, 安田講堂

HIrofumi Shoun
Direct Hydride Transfer from NADH to Cytochrome P450nor
The 50th Anniversary of Oxygenases: Advances and Reflections 〜「酵素添加酵素」の50年ー展望と回顧ー " Invited Speaker "
10-12 April 2006, Kyoto JAPAN

祥雲 弘文
「タンパク質構造解析に関する重要課題(P450を中心に)を説明」
2006年度日本農芸化学会大会シンポジウム「我が国の生物生産研究の課題と研究戦略〜農芸化学と社会貢献〜 共催:(独)科学技術振興機構
28th March 2006, Kyoto JAPAN

祥雲 弘文
「シトクロムP450の基礎とその魅力・潜在性に関して」
花王株式会社生物科学研究所
13 Feburary 2006, Tochigi JAPAN 


2005

Original Papers

Planifilum fimeticola gen. nov., sp. nov. and Planifilum fulgidum sp. nov., novel members of the family 'Thermoactinomycetaceae' isolated from compost
K. Hatayama, H. Shoun, Y. Ueda, and A. Nakamura
Int J Syst Evol Microbiol. 55 (5), 2101-2104 (2005)

Pseudomonas azotifigens sp. nov., a novel nitrogen-fixing bacterium isolated from a compost pile
K. Hatayama, S. Kawai, H. Shoun, Y. Ueda, and A. Nakamura
Int J Syst Evol Microbiol. 55 (4),1539-1544 (2005)

Gene expression and characterization of two 2-oxoacid:ferredoxin oxidoreductases from Aeropyrum pernix K1
Y. Nishizawa, T. Yabuki, E. Fukuda, and T. Wakagi
FEBS Lett. 579 (11), 2319-5322 (2005)

A series of crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with various cleavage products
S. Fushinobu, S.-Y. Jun, M. Hidaka, H. Nojiri, H. Yamane, H. Shoun, T. Omori, and T. Wakagi
Biosci. Biotechnol. Biochem. 69 (3), 491-498 (2005)

    Crystal structure of the terminal cumene dioxygenase component of Pseudomonas fluorescens IP01
    X. Dong, S. Fushinobu, E. Fukuda, T. Terada, S. Nakamura, K. Shimizu, H. Nojiri, T. Omori, H. Shoun, and T. Wakagi
    J. Bacteriol. 187 (7), 2483-2490 (2005)

    Cytochrome aa3 in facultatively aerobic and hyperthermophilic archaeon Pyrobaculum oguniense
    T. Nunoura, Y. Sako, T. Wakagi, and A. Uchida
    Can J Microbiol. 51 (8), 621-627 (2005)

    Crystal structure of the ferredoxin component of carbazole 1,9a-dioxygenase of Pseudomonas resinovorans strain CA10, a novel Rieske non-heme iron oxygenase system
    J.-W. Nam, H. Noguchi, Z. Fujimoto, H. Mizuno, Y. Ashikawa, M. Abo, S. Fushinobu, N. Kobashi, T. Wakagi, K. Iwata, T. Yoshida, H. Habe, H. Yamane, T. Omori, and H. Nojiri
    PROTEINS: Struct. Funct. Bioinform. 58 (4), 779-789 (2005)

    Structural basis for the specificity of the reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125
    S. Fushinobu, M. Hidaka, Y. Honda, T. Wakagi, H. Shoun, and M. Kitaoka
    J. Biol. Chem. 280 (17), 17180-17186 (2005)

    Crystallization and preliminary X-ray analysis of reducing-end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125
    Y. Honda, S. Fushinobu, M. Hidaka, T. Wakagi, H. Shoun, and M. Kitaoka
    Acta Cryst. F61 (3), 291-292 (2005)

    Expression of acetylcholine (ACh) and ACh-synthesizing activity in Archaea
    T. Yamada, T. Fujii, T. Kanai, T. Amo, T. Imanaka, H. Nishimasu, T. Wakagi, H. Shoun, M. Kamekura, Y. Kamagata, T. Kato, and K. Kawashima
    Life Sci. 77 (16), 1935-1944 (2005)

    Crystal structure of tetrameric homoisocitrate dehydrogenase from an extreme thermophile, Thermus thermophilus: Involvement of hydrophobic dimer-dimer interaction in extremely high thermotolerance
    J. Miyazaki, K. Asada, S. Fushinobu, T. Kuzuyama, and M. Nishiyama
    J. Bacteriol. 187 (19), 6779-88 (2005)

    Crystal structure of NAD-dependent malate dehydrogenase complexed with NADP(H)
    T. Tomita, S. Fushinobu, T. Kuzuyama, and M. Nishiyama
    Biochem. Biophys. Res. Comm. 334 (2), 613-618 (2005)

    Distinct conformation-mediated functions of an active site loop in the catalytic reactions of NAD-dependent D-lactate dehydrogenase and formate dehydrogenase
    T. Shinoda, K. Arai, M. Shigematsu-Iida, Y. Ishikura, S. Tanaka, T. Yamada, M. S. Kimber, E. F. Pai, S. Fushinobu, and H. Taguchi
    J. Biol. Chem. 280 (17), 17068-17075 (2005)

    Role of the conserved arginine residue in the loop 4 of glycoside hydrolase family 10 xylanases
    M. Nishimoto, M. Kitaoka, S. Fushinobu, and K. Hayashi
    Biosci. Biotechnol. Biochem. 69 (5), 904-910 (2005)

    Biochemical characterization of recombinant acetyl xylan esterase from Aspergillus awamori expressed in Pichia pastoris: Mutational analysis of catalytic residues
    T. Koseki, Y. Miwa, S. Fushinobu, and K. Hashizume
    Biochim. Biophys. Acta - Proteins Proteomics 1749 (1), 7-13 (2005)

    Mutational analysis of a feruloyl esterase from Aspergillus awamori involved in substrate discrimination and pH dependence
    T. Koseki, K. Takahashi, S. Fushinobu, H. Iefuji, K. Iwano, K. Hashizume, and H. Matsuzawa
    Biochim. Biophys. Acta - Gen. Subjects 1722 (2), 200-208 (2005)

Others

Flavohemoglobin and NO-related enzymes in nitrogen cycle
H. Shoun, pp. 799-807
Interaction of cytochrome P450foxy with fatty acids
M. Kobayashi, S. Fushinobu, T. Wakagi, and H. Shoun, pp. 449-451
In Flavins and Flavoproteins 2005 (Proceedings of the 15th International Symposium on Flavins and Flavoproteins; T. Nishino, R. Miura, M. Tanokura and K. Fukui, eds), ARchiTect inc., Tokyo, Japan

Nitric oxide reductase (P450(nor)) from Fusarium oxysporum
A. Daiber, H. Shoun, and V. Ullrich
J. Inorg. Biochem. 99 (1),185-193 (2005)

低酸素環境下でのカビの呼吸と発酵
高谷直樹、祥雲弘文
バイオサイエンスとインダストリー 63 (4), 233-236 (2005)
Anaerobic respiration and fermentation by filamentous fungi
N. Takaya and H. Shoun
Bioscience and Industry 63 (4), 233-236 (2005) [Japanese]

GH-94加リン酸分解酵素の反応機構と基質認識
日高將文、本多裕司、北岡本光、韮沢悟、林清、若木高善、祥雲弘文、伏信 進矢
(第12回糖質関連酵素化学シンポジウム)
応用糖質科学 52 (2), 191-196 (2005)
Reaction mechanism and substrate recognition of GH-94 phosphorolytic enzymes
M. Hidaka, Y. Honda, M. Kitaoka, S. Nirawasa, K. Hayashi, T. Wakagi, H. Shoun, and S. Fushinobu
(Proceedings of the Symposium on Amylases and Related Enzymes, 2004)
J. Appl. Glycosci. 52 (2), 191-196 (2005) [Japanese]

Reclassification of inverting phosphorylases based on structure determination
M. Hidaka, Y. Honda, M. Kitaoka, T. Wakagi, H. Shoun, and S. Fushinobu
Photon Factory Activity Report #22 2004 Part A, Highlights [Biological Science], pp. 43-44

Lectures etc.

Hirofumi Shoun
Involvement of Flavohemoglobin and Nitric oxide in the Nitrogen Respiration by the Bacterium Streptomyces antibioticus
15th International Symposium on Flavins and Flavoproteins " Invited Speaker "
17-22 April 2005, Shonan KANAGAWA