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1995 1994 1993
Reddish Escherichia coli cells caused by overproduction of Bacillus stearothermophilus uroporphyrinogen III methylase: Cloning, sequencing, and expression of the gene
P.-L. Chou, M. Ohtsuka, T. Minowa, K. Yamasato, Y. Sakano, H. Matsuzawa, T. Ohta, and H. Sakai
Biosci. Biotech. Biochem. 59, 1817-1824 (1995)Comparison of primary structures and substrates specificities of two pullulan-hydrolyzing alpha-amylases, TVA-I and TVA-II, from Thermoactinomyces vulgaris R-47
T. Tonozuka, S.-I. Mogi, Y. Shimura, A. Ibuka, H. Sakai, H. Matsuzawa, Y. Sakano, and T.Ohta
Biochim. Biophys. Acta 1252 (1), 35-42 (1995)Molecular cloning of the genes for pyruvate kinase of Two Bacilli, Bacillus psychrophilus and Bacillus licheniformis, and comparison of the properties of the enzymes produced in Escherichia coli
K. Tanaka, H. Sakai, T. Ohta, and H. Matsuzawa
Biosci. Biotech. Biochem. 59, 1536-1542 (1995)Expression of Aqualysin-I (a thermophilic protease) in soluble form in Escherichia coli under a Bacteriophage T7 Promoter
S. Sakamoto, I. Terada, M. Iijima, T. Ohta, and H. Matsuzawa
Biosci. Biotechnol. Biochem. 59 (8), 1438-1443 (1995)Cloning and sequence of the gene encoding a cefotaxime-hydrolyzing class A beta-lactamase isolated from Escherichia coli
Y. Ishii, A. Ohno, H. Taguchi, S. Imajo, M. Ishiguro, and H. Matsuzawa
Antimicrob. Agents Chemother. 39 (10), 2269-2275 (1995)Crystallization and preliminary X-ray analysis of Thermoactinomyces vulgaris R-47 alpha-amylase-II
S. Kamitori, T. Satou, T. Tonozuka, Y. Sakano, H. Matsuzawa, and K. Okuyama
J. Struct. Biol. 114 (3), 229-231 (1995)
T and R states in the crystals of bacterial L-lactate dehydrogenase reveal the mechanism for allosteric control
S. Iwata, K. Kamata, S. Yoshida, T. Minowa, and T. Ohta
Nat. Struct. Biol. 1 (3), 176-185 (1994) Cover Illustration of The IssueA convenient enzymatic synthesis of 4(2)-alpha-isomaltosylisomaltose using Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II)
T. Tonozuka, H. Sakai, T. Ohta, and Y. Sakano
Carbohydrate Res. 261, 157-162 (1994)Fermentation conditions for efficient production of thermophilic protease in Escherichia coli harboring a plasmid.
S. Sakamoto, I. Terada, M. Iijima, et al.
Appl. Microbiol. Biotechnol. 42, 569-574 (1994)Production of thermophilic protease by glucose-controlled fed-batch culture of recombinant Escherichia coli.
S. Sakamoto, M. Iijima, et al.
J. Ferment. Bioeng. 78 (4), 304-309 (1994)Carboxypeptidase Taq, a thermostable zinc enzyme, from Thermus aquaticus YT-1: Molecular cloning, sequencing, and expression of the encoding gene in Escherichia coli.
S.-H. Lee, H. Taguchi, E. Yoshimura, et al.
Biosci. Biotech. Biochem. 58 (8), 1490-1495 (1994)Requirement of a COOH-terminal pro-sequence for the extracellular secretion of aqualysin I (a thermostable subtilisin-type protease) in Thermus thermophilus.
Y.-C. Lee, H. Koike, H. Taguchi, et al.
FEMS Microbiol. Lett. 120 (1-2), 69-74 (1994)Functional roles of Trp337 and Glu632 in Clostridium glucoamylase, as determined by chemical modification, mutagenesis, and the stopped-flow method.
H. Ohnishi, H. Matsumoto, H. Sakai, and T. Ohta
J. Biol. Chem. 269 (5), 3503-3510 (1994)Essential role of arginine 235 in the substrate-binding of Lactobacillus plantarum D-lactate dehydrogenase.
H. Taguchi, and T. Ohta
J. Biochem. 115 (5), 930-936 (1994)A regular 1:1 complex of two allosteric states in the single crystal of L-lactate dehydrogenase from Bifidobacterium longum.
S. Iwata, S. Yoshida, and T.Ohta
J. Mol. Biol. 236 (3), 958-959 (1994)
Identification of glutamic acid 204 and aspartic acid 200 in chitinase A1 of Bacillus circulans WL-12 as essential residues for chitinase activity.
T. Watanabe, K. Kobori, K. Miyashita, T. Fujii et al.
J. Biol. Chem., 268 (25), 18567-18572 (1993)A neopullulanase-type a-amylase gene from Thermoactinomyces vulgaris R-47.
T. Tonozuka, M. Ohtsuka, S. Mogi, H. Sakai et al.
Biosci. Biotech. Biochem., 57 (3), 395-401 (1993)Molecular cloning and nucleotide sequence of the gene for pyruvate kinase of Bacillus stearothermophilus and the production of the enzyme in Escherichia coli: Evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon.
H. Sakai and T. Ohta
Eur. J. Biochem., 211 (3), 851-859 (1993)Purification, crystallization and preliminary X-ray investigation of Aqualysin I, a heat stable serine protease
P. R. Green, J. D. Oliver, L. C. Strickland, D. R. Toerner,H. Matsuzawa, and T. Ohta
Acta Crystallogr. D49, 349-352 (1993)Histidine 296 is essential for the catalysis in Lactobacillus plantarum D-lactate dehydrogenase.
H. Taguchi and T. Ohta
J. Biol. Chem. 268 (24),18030-4 (1993)Molecular basis of allosteric activation of bacterial L-lactate dehydrogenase.
S. Iwata and T. Ohta
J. Mol. Biol. 230 (1), 21-27 (1993)