宮永 顕正 准教授
Akimasa MIYANAGA, Ph.D.
E-mail: amiyanaga[at]g.ecc.u-tokyo.ac.jp
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教員カタログ

進めている研究について
放線菌などの微生物が生産する天然有機化合物は多様な骨格構造、多様な生物活性を有しており、抗生物質や医薬品として使用されているものが多く存在します。我々は、天然有機化合物の複雑な炭素骨格が構築される仕組みに興味を持ち、その生合成に関わる酵素群の構造解析や生化学的解析を進めています。



研究手法について
X線結晶構造解析、クライオ電子顕微鏡解析、タンパク質工学的手法、各種分光学的手法などを組み合わせて研究を行っています。


代表的な論文
(全業績: Google Scholar、Web of Science、ORCiD)

  • Ancestral sequence reconstruction as a tool for structural analysis of modular polyketide synthases
    Chisuga, T., Takinami, S., Liao, Z., Karasawa, M., Adachi, N., Kawasaki, M., Moriya, T., Senda, T., Terada, T., Kudo, F., Eguchi, T., Nakano, S.*, Ito, S.*, and Miyanaga, A.*
    Nat. Commun. 16, 6847 (2025)

  • Elucidation of interface interactions between a dehydratase domain and an acyl carrier protein in cremimycin polyketide synthase
    Kotagiri, K., Tachibana, H., Kawasaki, D., Chisuga, T., Kashima, T., Fushinobu, S., Kudo, F., Eguchi, T.*, and Miyanaga, A.*
    FEBS Lett. 599, 1159-1168 (2025) Link

  • Structural basis of amide-forming adenylation enzyme VinM in vicenistatin biosynthesis
    Miyanaga, A.*, Nagata, K., Nakajima, J., Chisuga, T., Kudo, F., and Eguchi, T.*
    ACS Chem. Biol. 18, 2343-2348 (2023) Link
    研究内容の説明

  • Structure-based analysis of transient interactions between ketosynthase-like decarboxylase and acyl carrier protein in a loading module of modular polyketide synthase
    Chisuga, T., Murakami, S., Miyanaga, A.*, Kudo, F., and Eguchi, T.*
    ACS Chem. Biol., 18, 1398-1404 (2023) Link
    研究内容の説明

  • Structural insight into the reaction mechanism of ketosynthase-like decarboxylase in a loading module of modular polyketide synthases
    Chisuga, T., Nagai, A., Miyanaga, A.*, Goto, E., Kishikawa, K., Kudo, F., and Eguchi, T.*
    ACS Chem. Biol., 17, 198-206 (2022) Link
    研究内容の説明

  • Structural characterization of complex of adenylation domain and carrier protein by using pantetheine cross-linking probe
    Miyanaga, A.*, Kurihara, S., Chisuga, T., Kudo, F., and Eguchi, T.*
    ACS Chem. Biol., 15, 1808-1812 (2020) Link

  • Structural basis of protein-protein interactions between a trans-acting acyltransferase and acyl carrier protein in polyketide disorazole biosynthesis
    Miyanaga, A.*, Ouchi, R., Ishikawa, F., Goto, E., Tanabe, G., Kudo, F., and Eguchi, T.*
    J. Am. Chem. Soc., 140, 7970-7978 (2018) Link

  • Structure-based analysis of the molecular interactions between acyltransferase and acyl carrier protein in vicenistatin biosynthesis
    Miyanaga, A.*, Iwasawa, S., Shinohara, Y., Kudo, F., and Eguchi, T.*
    Proc. Natl. Acad. Sci. USA, 113, 1802-1807 (2016) Link

  • The crystal structure of the adenylation enzyme VinN reveals a unique β-amino acid recognition mechanism
    Miyanaga, A., Cieślak, J., Shinohara, Y., Kudo, F., and Eguchi, T.*
    J. Biol. Chem., 289, 31448-31457 (2014) Link

  • Flavin-mediated dual oxidation controls an enzymatic Favorskii-type rearrangement
    Teufel, R.#, Miyanaga, A.#, Michaudel, Q.#, Stull, F.#, Louie, G., Noel, J. P., Baran, P. S., Palfey, B., and Moore, B. S.*
    Nature, 503, 552-556 (2013) Link
    新着論文レビュー

  • Direct transfer of starter substrates from type I fatty acid synthase to type III polyketide synthases in phenolic lipid synthesis
    Miyanaga, A., Funa, N., Awakawa, T., and Horinouchi, S.*
    Proc. Natl. Acad. Sci. USA, 105, 871-876 (2008) Link
    研究科研究成果

  • Crystal structure of a family 54 α-L-arabinofuranosidase reveals a novel carbohydrate-binding module that can bind arabinose
    Miyanaga, A., Koseki, T., Matsuzawa, H., Wakagi, T., Shoun, H., and Fushinobu, S.*
    J. Biol. Chem., 279, 44907-44914 (2004) Link


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