2000-2002 Publications etc.
2002 2001 2000
平成14年度有馬啓記念バイオインダストリー協会賞
(財) バイオインダストリー協会
真菌脱窒系の発見とP450norの構造・機能相関解明
酵素学研究室教授 祥雲 弘文
Professor Kei Arima Memorial Award, Japan Bioindustry Association, 2002
Dicovery of the Fungal Denitrifying System and Studies on Structure-Function Relationship of P450nor
Prof. SHOUN, Hirofumi・日経産業新聞にバイオ協会賞受賞について掲載 2002年8月5日
・JBAニュースにて紹介 バイオサイエンスとインダストリー Vol.60 No.9 2002
・受賞についてのインタビュー記事掲載 Science & Technology Journal p62 : Dec. 2002
･ 真菌脱窒系の発見とP450norの構造・機能相関解明
バイオサイエンスとインダストリー 61, 11-16 (2003)
Genetic engineering using fungal flavohemoglobin for constructing Pseudomonas stutzeri strain emitting less nitrous oxide
N. Takaya, H. Shoun
J. Biosci. Bioeng. 94: (3), 282-284 (2002)The B' helix determines cytochrome P450nor specificity for the electron donors NADH and NADPH
L. Zhang, T. Kudo, N. Takaya, and H. Shoun
J. Biol. Chem. 277 (37), 33842-33847 (2002)Ammonia fermentation, a novel anoxic metabolism of nitrate by fungi
Z. Zhou, N. Takaya, A. Nakamura, M. Yamaguchi, K. Takeo, and H. Shoun
J. Biol. Chem. 277 (3), 1892-1896 (2002)Nitrate reductase-formate dehydrogenase couple involved in the fungal denitrification by Fusarium oxysporum
H. Uchimura, H. Enjoji, T. Seki, A. Taguchi, N. Takaya, and H. Shoun
J. Biochem. 131 (4), 579-586 (2002)Codenitrification and denitrification are dual metabolic pathways by which dinitrogen evolves from nitrate in Streptomyces antibioticus
Y. Kumon, Y. Sasaki, I. Kato, N. Takaya, H. Shoun, and T. Beppu
J. Bacteriol. 184 (11), 2963-2968 (2002)Kinetic analysis on hydroxylation of saturated fatty acids by recombinant P450foxy produced by Escherichia coli espression system.
T. Kitazume, A. Tanaka, N. Takaya, A. Nakamura, S. Matsuyama, T. Suzuki, and H. Shoun
Eur. J. Biochem. 269 (8), 2075-2082 (2002)Isotope effects and intermediates in the reduction of NO by P450nor.
A. Daiber, T. Nauser, P. Weber, C. Hultschig, N. Takaya, H. Shoun, and V. Ullrich
J. Inorg. Biochem. 88 (3-4), 343-352 (2002)Transcriptional control of nitric oxide reductase gene (CYP55) in the fungal denitrifier Fusarium oxysporum
N. Takaya, H. Uchimura, and H. Shoun
Biosci. Biotech. Biochem. 66 (5), 1039-1045 (2002)Well maintained CYP genes in sandwich-culturing hepatocytes: quantitative analysis using real-time PCR method
N. Toritsuka, Y. Nonogaki, Y. Hori, S. Sawada, H. Shoun, and S. Motooka
Res. Commun. Mol. Pathol. Pharmacol. (2002)Crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with cleavage products
S. Fushinobu, T. Saku, M. Hidaka, S.-Y. Jun, H. Nojiri, H. Yamane, H. Shoun, T. Omori, and T. Wakagi
Protein Science 11(9), 2184-2195 (2002)Advantages of sandwich-culturing hepatocytes for assessment of chronic hepatotoxicity.
N. Toritsuka, Y. Nonogaki, Y. Hori, T. Sugihara, S. Sawada, H. Shoun, and S. Motooka
Res. Commun. Pharmacol. Toxicol. (2002)Trimeric crystal structure of the glycoside hydrolase family-42 beta-galactosidase from Thermus thermophilus A4 and the Structure of its Complex with Galactose
M. Hidaka, S. Fushinobu, N. Ohtsu, H. Motoshima, H. Matsuzawa, H. Shoun, T. Wakagi
J. Mol. Biol. 322 (1), 79-91 (2002)
カビと放線菌の新規窒素呼吸系
祥雲弘文
バイオサイエンスとインダストリー 60 (7), 11-15 (2002)
Novel nitrogen respiration by fungi and actinomycetes
Bioscience and Industry 60 (7), 11-15 (2002) [Japanese]Thermus thermophilus A4株由来・新規耐熱性βガラクトシダーゼおよび基質複合体のX線結晶構造解析
（糖質関連酵素化学シンポジウム）
日高將文、伏信 進矢、大津奈穂美、元島英雅、松澤洋、祥雲弘文、若木高善
応用糖質科学 49 (2), 175-180 (2002)
X-ray crystallography of a novel thermostable beta-galactosidase from Thermus thermophilus A4, and its complex structure with galactose
(Proceedings of the Symposium on Amylases and Related Enzymes, 2001)
M. Hidaka, S. Fushinobu, N. Ohtsu, H. Motoshima, H. matsuzawa, H. Shoun, and T. Wakagi
J. Appl. Glycosci. 49 (2), 175-180 (2002) [Japanese]Molecular mechanism of nitric oxide reduction catalyzed by fungal nitric oxide reductase.
Obayashi, E., shimizu, H., Park, s.-Y., Nakamura, H., Takahashi, S., Shoun, H., and Shiro, Y
Oxygen and Life: Oxygenases, Oxidases and Lipid Mediators; Excerpta Medica International Congress Series 1233 (Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F., eds) pp. 59-62, Elsevier, (2002)Cytochromes P450nor and P450foxy of the fungus Fusarium oxysporum.
Shoun, H., and Takaya, N.
Oxygen and Life: Oxygenases, Oxidases and Lipid Mediators; Excerpta Medica International Congress Series 1233 (Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F., eds) pp. 89-97, Elsevier, (2002)Analysis of fungal P450foxy (CYP505), a fused protein of cytochrome P450 and its reductase.
Kitazume, T., Tanaka, A., Matsuyama, S., Takaya, N., and Shoun, H.
Oxygen and Life: Oxygenases, Oxidases and Lipid Mediators; Excerpta Medica International Congress Series 1233 (Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F., eds) pp. 143-149, Elsevier, (2002)Distribution, structure and function of fungal nitric oxide reductase P450nor- recent advances.
Zhang, L., Kudo, T., Takaya, N., and Shoun, H.
Oxygen and Life: Oxygenases, Oxidases and Lipid Mediators; Excerpta Medica International Congress Series 1233 (Ishimura, Y., Nozaki, M., Yamamoto, S., Shimizu, T., Narumiya, S., and Mitani, F., eds) pp. 89-97, Elsevier, (2002)Nitric Oxide synthesis by the Actinomycete S.antibioticus.
Yasuyuki Sasaki, Naoki Takaya, Akira Nakamura, Hirofumi Shoun
Arch. Biochem. Biophys., Part B : NITRIC OXIDE, Biology and Chemistry 6, 459-460(2002)Binding of NAD(P)H to NO reductase Cytochrome P450nor
Hirofumi Shoun, Li Zhang, Takashi Kudo, Fei Su, Naoki Tkaya
Arch. Biochem. Biophys., Part B : NITRIC OXIDE, Biology and Chemistry 6, 463-463(2002)Fungal cytochrome P450nor and flavohemoglobin are involved in survival under NO stress.
Naoki Takaya, Hidetoshi Morita, Hirofumi Shoun
Arch. Biochem. Biophys., Part B : NITRIC OXIDE, Biology and Chemistry 6, 472-472(2002)
｢カビ Fusarium oxysporum の P450nor と P450foxy」
1st Workship on P450 Biodiversity and Biotechnology
29-30 November 2002, awajiyumebutai, Japan「亜酸化窒素（N20)抑止型好気脱窒システムの構築と水処理への応用」
主催：JST 科学技術振興事業団 Japan Science and Technology Corporation
26 November, 2002, Tokyo, Japan真菌脱窒系の発見とP450norの構造・機能相関説明
財団法人化学技術戦略推進機構 (JCII) にて
20 November 2002, Tokyo, Japan｢真菌脱窒系の発見とP450norの構造・機能相関解明
バイオインダストリー協会賞受賞記念講演
24 October 2002, Tokyo, Japan｢Application of p450nor and P450foxy to Green Chemistry｣
Korea-Japan／Japan-Korea Biotechnology Industry Investment & Cooperation Conference ＜韓日 生物産業交流協力機構＞
30 September - 2 October 2002, Seoul, Korea｢Diversified functions and application of cytochrome P450｣
10th Anniversary Novozymes Japan Enzyme Symposium
27 September 2002, Kyoto, Japan特別講演「地球環境と微生物バイオテクノロジー」
金沢大学 集中講義「微生物を利用したバイオテクノロジー」
17-18 September 2002, Kanazawa, Japan2nd International Conference, Biology,Chemistry and Therapeutic Applications of Nitric Oxide ( NO 2002 ) ＜第２回国際NO会議>
16-20 June 2002, Prague, Czech Republic
Cytochromes p450 and flavin monooxygenases-targets and sources of nitric oxide.
Morgan ET, Ullrich V, Daiber A, Schmidt P, Takaya N, Shoun H, McGiff JC, Oyekan A, Hanke CJ, Campbell WB, Park CS, Kang JS, Yi HG, Cha YN, Mansuy D, Boucher JL.:
Drug Metab. Dispos. 29,1366-76 (2001)Purification and cDNA cloning of nitric oxide reductase cytochrome P450nor (CYP55A4) from Trichosporon cutaneum
Zhang, L., Takaya, N., Kitazume, T., Kondo, T., and Shoun, H.
Eur. J. Biochem. 268, 3198-3204, (2001)A novel C1-using denitrifier Alcaligenes sp STC1 and its genes for copper-containing nitrite reductase and azurin
Ozeki, S., Baba, I., Takaya, N., and Shoun, H.
Biosci. Biotech. Bioch., 65, 1206-1210, (2001)Involvement of formate as an interspecies electron carrier in a syntrophic acetate-oxidizing anaerobic microorganism in coculture with methanogens
Hattori, S., Hongwei, L., Shoun, H., and Kamagata, Y.
J. Biosci. Bioeng., 91, 294-298, (2001)Oxygen requirement for denitrification by the fungus Fusarium oxysporum
Zhou, Z., Takaya, N., Sakairi, M.A.C., and Shoun, H.
Arch. Microbiol., 175, 19-25, (2001)A positive charge cluster formed in the heme-distal pocket of cytochorome P450nor is essential for interaction with NADH.
Kudo, T., Takaya, N., Park, S.-Y., Shiro, Y., and Shoun, H.
J. Biol. Chem., 276, 5020-5026 (2001)Advantages of sandwich-culturing hepatocytes for assessment of chronic hepatotoxicity.
Toritsuka, N., Nonogaki, Y., Hori, Y., Sugihara, T., Sawada, S., Shoun, H. and Motooka, S. Research Communications in Pharmacology and Toxicology 6, 327-338 (2001)
｢Cytochrome P450 as the materials for made - to - order enzymes｣
The 11th German - Japanese Workshop on Enzyme Technology
5-6 October 2001, Dresden, Germany｢Fungal denitrification and nitric oxide (NO) reductase P450nor｣
The 33th Symposium of Korean Society of Life Science-The Vision of Life Science 21 Century- 基調講演
14 September 2001, Seoul, Korea
Proton delivery in NO reduction by fungal nitric-oxide reductase. Cryogenic crystallography, spectroscopy, and kinetics of ferric-NO complexes of wild-type and mutant enzymes.
Shimizu, H., Obayashi, E., Gomi, Y., Arakawa, H., Park, S.-Y., Nakamura, H., Adachi, S., Shoun, H., and Shiro, Y.
J. Biol. Chem., 276, 4816-4826 (2000)Crystal structures of cytochorome P450nor and its mutants (Ser286 -> Val, Thr) in the ferric resting state at cryogenic temperature: a comparative analysis with monooxygenase cytochorome P450s.
Shimizu, H., Park, S.-Y., Lee, D.-S., Shoun, H., Shiro Y.
J. Inorg. Chem., 81, 191-205 (2000)Mutation effects of a conserved thereonine (Thr243) of cytochorome P450nor on its structure and function.
Obayashi, E., Shimizu, H., Park, S.-M., Shoun, H., and Shiro, Y.
J. Inorg. Chem., 82, 103-111 (2000)Fusarium oxysporum fatty-acid subterminal hydroxylase (CYP505) is a membrane-bound eukaryotic counterpart of Bacillus megaterium cytochorome P450BM3.
Kitazume, T., Takaya, N., Nakayama, N., and Shoun, H.
J. Biol. Chem., 275, 39734-39740 (2000)Nitric oxide reduction, the last step in denitrification by Fusarium oxysporum, is obligatorily mediated by cytochorome P450nor.
Takaya, N., Shoun, H.,
Mol. Gen. Genet., 263, 342-348 (2000)Thermacetogenium phaeum gen. nov., sp nov., a strictly anaerobic, thermophilic, syntrophic acetate-oxidizing bacterium.
Hattori, S., Kamagata, Y., Hanada, S., and Shoun, H.
Int. J. Syst. Evol. Microbiol., 50, 1601-1609, (2000)Rapid reactions of peroxynitrite with heme-thiolate proteins as the basis for protection of prostacyclin synthase from inactivation by nitration.
Zou MH, Daiber A, Peterson JA, Shoun H, Ullrich V.
Arch. Biochem. Biophys., 376, 149-155, (2000)