Research Subject 1:

Energy metabolic systems of (hyper)thermophilic archaea (Fig. 1 and Fig. 2) and bacteria

1. 4-alpha-Glucanotransferase
2. ADP-dependent glucokinase (Fig. 3) and phosphofructokinase
3. ATP-dependent hexokinase (Fig.4)
4. Iron-containing cupin-type phosphoglucoisomerase
5. Fructose bisphosphatase
6. 2-Oxoacid:ferredoxin oxidoreductase and zinc-containing ferredoxin (Fig. 5)
7. Respiratory electron transport system of aerobic archaea
8. Cytosolic membrane proteins and cell surface proteins
9. Huge enzyme involved in oxidation and reduction of elemental sulfur

Research Subject 2:

Structure and function of industrial enzymes

Fig. 1 Phylogenetic tree of life based on the SS rRNA

Fig. 2 Electron microscopic image of a thermoacidophilic archaeon Sulfolobus tokodaii

Fig. 3 Structure of ADP-dependent glucokinase from a hyperthermophilic archaeon Thermococcus litoralis

Fig. 4 A large conformational change of hexokinase from a thermoacidophilic archaeon S. tokodaii, which is induced by a sugar substrate binding

Fig. 5 Structure of ferredoxin from S. tokodaii

E-mail: t-wakagi[at-mark]cb3.so-net.ne.jp