The reaction of this enzyme is thought to proceed through two pathways: via ATP-bound form or via Glcose(Glc)-bound form. Here we show the movies of both possible pathways according to our crystal structures in four different forms. In both pathways, binding of Glc induces large strucrural change (domain closure).
For details, see our article on JBC.
Nishimasu et al., J. Biol. Chem. 282 (13), 9923-9931 (2007)
via ATP-bound form QuickTime movie (320 KB) Animation GIF (1.7 MB)
|
State
|
E
|
 |
E:ATP
|
|
E:ATP:Glc
|
|
E:ATP:Mg2+:Glc
|
|
E:ADP:Mg2+:Glc-1-P
|
|
|
Open
|
+ ATP
|
Open
|
+ Glc
|
Closed
|
+ Mg2+
|
Closed
|
(catalysis)
|
Closed
|
(release of product)
|
|
|
Crystal
Structure |
E
|
E:ADP
|
Domain
Closure |
E:ADP:Mg2+:Xyl
|
via Glc-bound form QuickTime movie (308 KB) Animation GIF (1.5 MB)
|
State
|
E
|
|
E:Glc
|
|
E:ATP:Mg2+:Glc
|
|
E:ADP:Mg2+:Glc-1-P
|
|
|
Open
|
+ Glc
|
Closed
|
+ ATP:Mg2+
|
Closed
|
(catalysis)
|
Closed
|
(release of product)
|
|
|
Crystal
Structure |
E
|
Domain
Closure |
E:Glc
|
E:ADP:Mg2+:Xyl
|
*Xyl=Xylose
H. Nishimasu, S. Fushinobu, H. Shoun, and T. Wakagi