CYP105P1 from the soil bacteria, Streptomyces, is involved in the biosynthesis of the antibiotic filipin, which is widely used as a diagnostic tool for a cholesterol disorder disease. Our group revealed a large structural change of this enzyme during the substrate binding.
High quality QuickTime movie (1.9 MB) is here.
CYP105P1 is a cytochrome P450 enzyme containing a heme (cyan) and an iron atom (orange). The hydroxylation of the substrate filipin I (green) takes place at the active site with a dioxygen and electrons. The electrons are delivered from a cytochrome P450 reductase.
For details of this research, see our articles on JB and JBC.
Xu et al., J. Bacteriol. 191 (4), 1211-1219 (2009)
Xu et al., J. Biol. Chem. 285 (22), 16844-16853 (2010)
L.-H. Xu, S. Fushinobu, T. Wakagi, and H. Shoun